The Biofabrication group develops new biofabrication technologies to generate libraries of 3D scaffolds able to control cell fate. This passes through the design of biomaterials, 3D scaffolds, and surface properties to better understand cell-material interactions. Our overarching goal is to create new solutions for regenerative medicine and understand the fundamental phenomena at the base of the observed regenerative processes.
The core activities of the group evolve around acquiring and implementing knowledge for biofabrication technologies based on the following research objectives:
- Design of scaffolds able to control and steer (stem) cell activity. Stem cells are a fascinating and promising source to regenerate tissues and organs due to their potential to differentiated into multiple specialized cells. Yet, better control over cell-material interactions is necessary to maintain tissue engineered constructs in time. It is crucial to control stem cell quiescence, proliferation and differentiation in three-dimensional scaffolds while maintaining cells viable in situ.
- Develop current and new biofabrication technologies based on additive manufacturing, bioprinting, bio-assembly, and electrospinning. Among biofabrication technologies, bioprinting, additive manufacturing, bio-assembly, and spinning technologies form crucial clusters that shall be used for this purpose. These technologies will be further advanced in the future to include surface engineering methods during fabrication.
- Integrate neural and vascular cues in tissue and organ regeneration strategies. Initial investigations on how different biofabrication platforms could be combined to recreate a synthetic mimicry of the ECM of the peripheral nervous system have been started. The goal in the coming years is to complement this know-how with vascularization and understand how neurovascular stimuli can modulate tissue regeneration.
- Engineer the immune response of biomaterials, scaffolds, and biomedical devices. Engineered devices with surface properties able to steer the foreign body response to synthesize a vascular graft for dialytic patients have already been successfully created. Further deepening our understanding of how biomedical implants can be engineered to steer the foreign body response is an exciting field in regenerative medicine as it will allow improving the integration of biofabricated substitutes with surrounding tissues.
- Apply biofabrication technologies to study regenerative and degenerative phenomena. 3D constructs could be used as 3D in vitromodels to understand biological mechanism behind tissue regeneration, homeostasis, and eventual degeneration. This will be fed back into the design of biofabricated constructs to achieve on one side a better 3D construct, on the other side possible new therapies for targeted diseases.
Funding sources and collaborations
The Biofabrication group gratefully acknowledges its funding sources, many of which have enabled the establishments of international collaborations in the form of consortia. Below, the main funders and consortia are depicted.
- Yao, T., Wieringa, P., Chen, H., Chandrakar, A., Samal, P., Giselbrecht, S., Baker, M. B., Moroni, L. (2020). Fabrication of a Self-assembled Honeycomb Nanofibrous Scaffold to Guide Endothelial Morphogenesis. Biofabrication. In press. https://doi.org/10.1088/1758-5090/ab9988
- Zonderland, J., Moldero, I. L., Anand, S., Mota, C., Moroni, L. (2020). Dimensionality changes actin network through lamin A/C and zyxin. Biomaterials. 240:119854. https://doi.org/10.1016/j.biomaterials.2020.119854
- Moroni, L., Burdick, J. A., Highley, C., Lee, S. J., Morimoto, Y., Takeuchi, S., & Yoo, J. J. (2018). Biofabrication strategies for 3D in vitro models and regenerative medicine. Nature reviews materials, 3(5), 21-37. https://doi.org/10.1038/s41578-018-0006-y
- Hendrikson, W. J., Rouwkema, J., Clementi, F., van Blitterswijk, C. A., Fare, S., & Moroni, L. (2017). Towards 4D printed scaffolds for tissue engineering: exploiting 3D shape memory polymers to deliver time-controlled stimulus on cultured cells. Biofabrication, 9(3), . https://doi.org/10.1088/1758-5090/aa8114
- Gunnewiek, M. K., Di Luca, A., Bollemaat, H. Z., van Blitterswijk, C. A., Vancso, G. J., Moroni, L., & Benetti, E. M. (2015). Creeping Proteins in Microporous Structures: Polymer Brush-Assisted Fabrication of 3D Gradients for Tissue Engineering. Advanced Healthcare Materials, 4(8), 1169-1174. https://doi.org/10.1002/adhm.201400797