Interreg-Biomat project update

Within the first two years of Biomat-on-Microfluidic Chip project, important steps towards creating a new generation of patient-specific organ-on-chip platforms were taken by developing several engineering and biological technologies:

• First designs and models of the chip have been made in the form of inserts for culture plates. We have succeeded in forming three-dimensional biological micro-constructs of patient-specific cells and various biomaterials at micron scale. In addition to that, we have developed multiple robust bioassays for screening the toxicity/biocompatibility and the functionality of newly developed biomaterials.
• Although this 3D model has been tested with human cell lines and stem cells, we have also optimized protocols for differentiating human-induced pluripotent stem cells (hiPSCs) towards muscle and bone tissues and for coupling these cells with reporters with which their growth and metabolism can be monitored non-invasively. Furthermore, we have shown the formation of 3D micro-tissues using these cells.
• The development of the platform for vascularization of 3D micro-tissues has also been demonstrated.
• In addition to that, we have developed a sensor that can be coupled to the chip in order to monitor tissue formation overtime.
• We have developed a technology for fabrication of micro-biomaterials with different chemistries that can be screened in the platform for biocompatibility and biological functionality.